Replication Machinery in Bacillus Subtilis

Replication Machinery in Bacillus Subtilis

Submitted by Melanie Berkmen of the Grossman Laboratory in the Department of Biology

MIT Department of Biology

Melanie Berkmen
Grossman Laboratory, Department of Biology
Epi-Fluorescence Micrograph

"This image shows simultaneous localization of the origin of replication and the replication machinery in live Bacillus subtilis cells.  Cell outlines (shown in white/grey) were visualized with the vital membrane stain FM4-64.  The origin region (cyan) was visualized using LacI-CFP bound to lac operators inserted near oriC. The replisome (yellow) was visualized using a fusion of the Tau subunit of DNA polymerase to YFP."

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